This study demonstrates that aerobic exercise, specifically swimming training, improves learning and memory in Alzheimer's disease model mice by enhancing glymphatic system function and promoting AQP4 polarization, which facilitates clearance of β-amyloid in the hippocampus. These findings suggest that exercise-induced upregulation of Lama1 and Dp71 supports glymphatic clearance mechanisms, thereby reducing Aβ deposition and cognitive impairment.