ROS are considered to be harmful when produced in excess and they are associated with the development of various diseases like Alzheimer's, Cancer, Infertility and Parkinson's. Oxidative stress biomarkers can be used to assess the severity of such diseases, while biochemical pathways and progression understanding can be used to develop new targets as prophylactic drugs. Thus, discovering biomarker responsible for the aetiology of disease could be helpful in understanding the course of disease.