The bioorthogonal ligation between isonitriles and azomethine imines (AMIs)—the AMI-isonitrile ligation—combines exquisite chemoselectivity with a stable ligation product, a small molecular reporter, and a pH-dependent rate. In this work, we tailored the modular structure of the dipolar AMI to increase Brønsted basicity and electrophilicity. These additive structural modifications increased the ligation rate by more than two orders of magnitude to 14 M⁻¹s⁻¹ at pH 7, 140 M⁻¹s⁻¹ at pH 6, and >1,000 M⁻¹s⁻¹ at pH 5.

A variety of rotary molecular machine prototypes powered by light, chemical energy, or electrons have been synthesized and their operation in solution, gels, or on surfaces has been demonstrated. However, little data regarding their performances have been disclosed. Here, we report on the synthesis of molecules incorporating a five-arm rotor and the direct measurement of the work required to block the rotation around the central atom.

Background: Ovarian cancer presents with non-specific symptoms that make early diagnosis challenging and the prognosis poor. Ovarian and endometrial cancers exhibit similar genomic mutations and biomarker profiles. Endogenous volatile organic compounds (VOCs) are products of metabolic activity. In cancer, metabolites increase due to tumour necrosis, leading to cancer-specific VOC patterns. The aim of this study was to evaluate VOC analyses in plasma as diagnostic tests for early diagnosis of ovarian and endometrial cancer.

Metalloproteins have evolved through the selective, strong, and precise interactions between metal ions and polypeptides. The coordination of metal ions to amino acid side chains is therefore a fundamental prerequisite for designing metalloproteins. Here, we present Metal-Installer, a user-friendly in silico tool that proposes probable mutation sites for metal ligation.