Air pollution and climate change are key global challenges for cities and both have large impacts on human health and economic development.
Background: Synthetic biology is an emerging multidisciplinary area of research with the potential to deliver various novel agrifood applications.
This study supports SDGs 3 and 6 by identifying passive chlorination at the point of collection as an effective and scalable strategy for reducing diarrhoea in children and improving access to safe and affordable drinking water in a low-income urban setting.
This study supports SDG 1, 3, and 6 and by assessing socioeconomic determinants of leprosy risk in over 33 milion Brazilian individuals and providing a robust assessment of the contribution of deprivation to the risk of leprosy, which is classified as a rare disease.
This review discusses established transgender individuals on hormones who have reached their desired post-pubertal phenotype.
The purpose of this study was to examine how innovative SDP organizations’ interactions with external stakeholders influence the social innovation process.
Background: Humanity's current use of resources is not in line with the goal of sustainable development. Climate change impact scenarios appear worryingly pessimistic.
Traumatic brain injury (TBI) is one the most common human afflictions, contributing to long-term disability in survivors.
The number of countries with a national development plan has more than doubled, from about 62 in 2006 to 134 in 2018.
High-throughput screening for drug discovery is increasingly utilizing cellular systems of high physiological relevance, such as patient primary cells and organoid cultures. We used 3D-cultured cystic fibrosis patient bronchial epithelial cells to screen for new small-molecule correctors of the disease-causing F508del mutation in CFTR. Impaired mucociliary clearance due to insufficient airway hydration is a hallmark of cystic fibrosis and we used a simple measure of surface liquid levels to quantify F508del CFTR correction in cultured bronchial epithelial cells. Two robust assay formats were configured and used to screen more than 100,000 compounds as mixtures or individual compounds in 96-well format. The corrector discovery success rate, as measured by the number of hits confirmed by an electrophysiology assay on patient primary bronchial epithelial cells, was superior to screens in cell lines expressing recombinant F508del CFTR. Several novel corrector scaffolds were discovered that when combined with the clinical corrector VX-809 delivered combination responses greater than double that of VX-809 alone. This work exemplifies the advantages of a disease-relevant readout and 3D-cultured patient primary cells for the discovery of new drug candidates.
